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About the RDP's Pyrosequencing Pipeline

The Ribosomal Database Project's Pyrosequencing Pipeline aims to simplify the processing of large 16S rRNA sequence libraries obtained through pyrosequencing. This site processes and converts the data to formats suitable for common ecological and statistical packages such as SPADE, EstimateS, and R.


HOVER over tool menu item for a popup description;
CLICK on the tool menu item to begin working with it.


NCBI/EBI Submission Tools:new


Data Processing Steps:


Formats for Common Programs:


Analysis Tools:


Miscellaneous Utilities:



Note: If you experience problems with the pyrosequencing pipeline, please contact us and we will help you get your sequences processed.

Pyro News

05/13/2012  All RDP Maintenance Complete
Thank you all for your patience this week while we installed some much needed hardware upgrades and server maintenance to help us serve you better....

05/10/2012  myRDP Scheduled Maintenance
myRDP will be down saturday afternoon for database maintenance.

05/09/2012  Maintenance Update (Con't)
All RDP sites are accessible. myRDP will be down for maintenance this evening between 5 and 6pm.

05/09/2012  Maintenance Update
Pyro, Fungene, and CME sites are back online. RDP will be down until late morning.

05/08/2012  RDP Maintence
All RDP Sites (rdp, pyro, fungene and cme sites) will be down for hardware upgrades this evening between 11pm and 1am.

04/20/2012  Pyro tool updates
R Formatter, FASTA Sequence Selection and Dereplicate

03/06/2012  Scripting RDP Tools
We do not allow scripting of our interactive tools. We provide SOAP services for all maj...

02/17/2012  Problems with loading, turnaround, jobs aborted?
RDP use has increased over 10-fold since November/December

02/10/2012  MSU Network Slowdown
You may experience slow or no connection to the RDP website

12/01/2011  RDP featured in Science Watch, Dec. 2011
Director James Cole answers questions about the RDP Classifier

Questions/comments: rdpstaff@msu.edu